Composite

Part:BBa_K238018:Design

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-09-22)


EF ligation with promotor and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2006
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
    Illegal NgoMIV site found at 443
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 594
    Illegal AgeI site found at 1131
    Illegal AgeI site found at 1592
    Illegal AgeI site found at 2034
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

the genes ech and fcs were provided by the Chris French lab from the University of Edinburgh

References